Design by Sixnet. Cookies help us deliver our services. By using our services, you agree to our use of cookies. Learn more I accept I refuse. In stock. Quantity The minimum purchase order quantity for the product is 1. Thank you for understanding.
Usage The product is intended for scientific research and development purposes only. Storage Of Lyophilised Peptides Peptides will arrive in lyophilised powder form. The same applies for all of our peptides. Tip: for smaller individual doses, it can be convenient to use more water for reconstitution. This makes it easier to accurately measure each dose, as the total volume of solution is greater. The following instructions use 1mL of bacteriostatic water to mix a TB 5mg vial.
Gather the above recommended items for mixing, along with the vial of Thymosin Beta Important: let the vial come to room temperature before beginning the mixing process. Remove caps from both the TB and Bacteriostatic Water vials and wipe each rubber stopper with the alcohol swab.
Use syringe to withdraw 1mL from the Bacteriostatic Water vial. Take filled syringe and inject bacteriostatic water into the TB vial. Aim for the sides of the vial, letting the water trickle down gently to the bottom. Injecting straight into the powder can damage the Thymosin molecules, as they are held together by fragile peptide bonds. Take out the syringe and delicately swirl the vial to dissolve any remaining bits of powder.
Then add water or buffer to the desired concentration. Dissolved peptides are less stable than the lyophilized dry powder. The solutions should be aliquoted before freezing to avoid thawing-refreezing cycles.
The stock solutions should be in dry organic solvents to avoid premature hydrolysis. The buffer of pH is considered as optimum for the stability of the aliquots. Why do the dissolved peptides lose their activities? How to prevent peptides from losing their bioactivities?
The oxidation of Methionine yielding the sulfoxide might be the main reason for losing the peptide activity. The rate of sulfoxide formation is sequence-dependent. The best way is to replace the Methionine with its stable isostere Nle. Sulfotyrosine-containing peptides may lose their activity due to the desulfation.
Are peptides containing free Cys supplied as monomers? Will the Cys-containing Peptides be easily oxidized? LifeTein provides Cys containing peptides as monomers unless specified otherwise. As air oxidation cannot be completely prevented, reducing the peptide before use by treatment with dithiothreitol DTT may be helpful to your experiment.
How to dissolve peptides containing disulfide bridges? How do I know if a cysteine in a peptide is free or oxidized? Basic buffers should be avoided for peptides containing disulfide bridges.
Peptides containing free thiol group may oxidize to form dimers or oligomers during storage, even as the lyophilized dry powder at a low temperature. The disulfide bond formation is rapid at neutral or slightly basic pH. Disulfide bridge formation is reversible. The disulfide bonds can be reduced at basic conditions using DTT.
The pH This oxidation can be reverted. Peptides containing two or more thiol moieties may yield a mixture of products upon oxidation. The pH 7. Hence, peptides containing free cysteines are best dissolved in degassed solvents, e. DMSO should be avoided, especially with peptide trifluoroacetates.
Some old peptides may require the addition of acetic acid for dissolution. Do not dissolve the amyloid peptides in PBS. Dry ice is not required for the shipment of peptides. The lyophilizates in vacuum sealed vials are sufficiently stable to tolerate days at ambient temperature. Upon receipt, the product should be stored at the recommended storage temperature of or Unfortunately, the C-terminal carboxylic group cannot be converted to a carboxamide once the peptide is synthesized.
The peptide amide has to be synthesized and cleaved in a different method. The impurity profile and especially the peptide content can vary from batch to batch, even if we use the same the standard protocols of synthesis and purification. The Research-grade peptides are for laboratory and research purposes only!
They may not be used as drugs for humans.
0コメント