How does dig labeling work

Anal Biochem, in press Google Scholar. Jessberger R, Berg P Repair of deletions and double-strand gaps by homologous recombination in a mammalian in vitro system. Kessler C Detection of nucleic acids by enzyme-linked immuno-sorbent assay ELISA technique: an example for the development of a novel non-radioactive labeling and detection system with high sensitivity. Kessler C The digoxigenin DIG technology — a survey on the concept and realization of a novel bioanalytical indicator system.

Synthesis and properties of the nucleotide compounds of the digoxigenin system and of photodigoxigenin. Schmitz GG, Walter T, Seibl R, Kessler C Non-radioactive labeling of oligonucleotides in vitro with the the hapten digoxigenin by tailing with terminal transferase.

Applications of the digoxigenin system. Tautz D, Pfeifle C A nonradioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback. Young WS, III Simultaneous use of digoxigenin-and radiolabeled oligodeoxyribonucleotide probes for hybridization histochemistry.

Neuropeptides Edinburgh — Google Scholar. Zuber U, Schumann W Tn5cos: a transposon for restriction mapping of large plasmids using phage lambda terminase. Ochs D Protein contaminants of sodium dodecyl sulfate-polyacrylamide gels. Personalised recommendations. Cite chapter How to cite?

ENW EndNote. Compared to internal, random digoxigenylated probes, Digoxigenin is located at the 3'-End only and less likely interferes with probe binding. For the preparation of labeled dsDNA complexes, label each complementary oligonucleotide separately and anneal them before use. Estimation of Digoxigenin labeling degree Quantification of Digoxigenin labeling degree is essential for reproducible downstream results. An oligonucleotide dilution series is immobilized on a positively-charged membrane Dot Blot followed by an indirect detection of the Digoxigenin moiety using an anti-Digoxigenin-alkaline phosphatase AP conjugate.

Recommended oligonucleotide starting amount for chemiluminescent detection: fmol The following reagent amounts 3. Sitemap Imprint Privacy Statement. All Products. Analogs and Derivatives of